LOCUS       40924_34929       14283 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPlatTET-gRNA2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, 
            Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I.
  TITLE     Targeted DNA demethylation in vivo using dCas9-peptide repeat and 
            scFv-TET1 catalytic domain fusions
  JOURNAL   Nat. Biotechnol. 34 (10), 1060-1065 (2016)
  PUBMED    27571369
REFERENCE   2  (bases 1 to 14283)
  AUTHORS   Hatada I, Morita S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2016) Contact:Izuho Hatada Gunma University, 
            Institute for Molecular and Cellular Regulation; 3-39-15 
            Showa-machi, Maebashi, Gunma 371-8512, Japan URL 
            :http://epigenome.dept.showa.gunma-u.ac.jp/~hatada/
REFERENCE   3  (bases 1 to 14283)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14283)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2016"; volume: "34"; issue: "10"; pages: 
            "1060-1065"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2016) Contact:Izuho Hatada Gunma University, Institute for 
            Molecular and Cellular Regulation"; volume: " 3-39-15 Showa-machi, 
            Maebashi, Gunma 371-8512, Japan URL :http"; pages: 
            "//epigenome.dept.showa.gunma-u.ac.jp/~hatada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        367..642
                     /label=chicken beta-actin promoter
     intron          643..1651
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1727..1747
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             1802..5905
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5909..5935
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5954..5974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5981..6001
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6065..6121
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6188..6244
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6311..6367
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6434..6490
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6557..6613
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6701..6757
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7505..7531
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             7622..8332
                     /codon_start=1
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     promoter        9994..11704
                     /label=CAG
                     /note="CMV early enhancer fused to modified chicken 
                     beta-actin promoter"
     promoter        11694..11785
                     /label=AmpR promoter
     promoter        11787..12144
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             12179..12970
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    13205..13252
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      13581..14169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      421 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      481 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      541 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      601 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct
      661 tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      721 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      781 gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag
      841 ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
      901 cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      961 tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     1021 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1081 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     1141 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
     1201 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     1261 gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1321 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1381 ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc
     1441 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1501 gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc ttcggggggg
     1561 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     1621 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct
     1681 gtctcatcat tttggcaaag aattcccccg cgatcgcgcc accatgccca agaagaagcg
     1741 caaggtggga cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg ggatcgccac
     1801 catggacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
     1861 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     1921 gcacagcatc aagaagaacc tgatcggcgc cctgctgttc gacagcggag aaacagccga
     1981 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     2041 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     2101 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     2161 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     2221 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     2281 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     2341 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     2401 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     2461 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa
     2521 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     2581 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     2641 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     2701 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     2761 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     2821 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     2881 cggctacatc gatggcggag ccagccagga agagttctac aagttcatca agcccatcct
     2941 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     3001 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     3061 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     3121 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     3181 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     3241 agtggtggac aagggcgcca gcgcccagag cttcatcgag cggatgacca acttcgataa
     3301 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     3361 gtacaacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     3421 gagcggcgag cagaaaaaag ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     3481 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     3541 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     3601 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     3661 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     3721 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     3781 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     3841 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     3901 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     3961 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     4021 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     4081 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     4141 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     4201 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     4261 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     4321 tatcgtgcct cagagctttc tgaaggacga ctccatcgat aacaaagtgc tgactcggag
     4381 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     4441 gaactactgg cgccagctgc tgaatgccaa gctgattacc cagaggaagt tcgacaatct
     4501 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     4561 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     4621 cactaagtac gacgagaacg acaaactgat ccgggaagtg aaagtgatca ccctgaagtc
     4681 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     4741 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     4801 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     4861 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     4921 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     4981 gcctctgatc gagacaaacg gcgaaacagg cgagatcgtg tgggataagg gccgggactt
     5041 tgccaccgtg cggaaagtgc tgtctatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     5101 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg acaagctgat
     5161 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     5221 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     5281 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     5341 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     5401 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     5461 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     5521 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     5581 acagcacaaa cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     5641 gatcctggcc gacgctaatc tggacaaggt gctgagcgcc tacaacaagc acagagacaa
     5701 gcctatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     5761 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     5821 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     5881 cgacctgtct cagctgggag gcgacgccta tccctatgac gtgcccgatt atgccagcct
     5941 gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt
     6001 ggacggcatt ggtagtggga gcaacggcag cagcggatcc aacggtccga ctgacgccgc
     6061 ggaagaggag cttctgagca aaaactatca cctcgaaaac gaggttgcgc gactgaagaa
     6121 aggaagcggg tccggtggaa gtggctccgg atctggaggt tctggcagcg gaggtagcgg
     6181 cagtggcgaa gagctcctta gtaagaacta tcatctggaa aatgaggtag cgcgcttaaa
     6241 gaaagggtcg ggaagtggcg gcagcggaag tgggagtgga gggagcggtt ctggcggttc
     6301 cggcagtgga gaggagttgc tgtctaagaa ctaccactta gaaaacgaag tcgcacggct
     6361 aaaaaaaggt tccggctccg gcggctccgg ttctggaagc gggggctcgg gatcaggtgg
     6421 atctggatca ggagaggaat tgctttccaa aaactaccac cttgagaatg aggtggccag
     6481 gttaaagaag gggagcggct cggggggtag tggatcgggg tcgggcgggt caggaagcgg
     6541 tggtagcgga tctggggagg agctgctctc gaagaattac catttggaga acgaagtggc
     6601 gagactaaag aagggaagcg gtagtggtgg ttcagggtct ggttcaggtg gcagtgggtc
     6661 tgggggctca gggtccggga cggccggcct cggaagcgga gctactaact tcagcctgct
     6721 gaagcaggct ggagacgtgg aggagaaccc tggacctagt accatgggcc ccgacatcgt
     6781 gatgacccag agccccagca gcctgagcgc cagcgtgggc gaccgcgtga ccatcacctg
     6841 ccgcagcagc accggcgccg tgaccaccag caactacgcc agctgggtgc aggagaagcc
     6901 cggcaagctg ttcaagggcc tgatcggcgg caccaacaac cgcgcccccg gcgtgcccag
     6961 ccgcttcagc ggcagcctga tcggcgacaa ggccaccctg accatcagca gcctgcagcc
     7021 cgaggacttc gccacctact tctgcgccct gtggtacagc aaccactggg tgttcggcca
     7081 gggcaccaag gtggagctga agcgcggcgg cggcggcagc ggcggcggcg gcagcggcgg
     7141 cggcggcagc agcggcggcg gcagcgaggt gaagctgctg gagagcggcg gcggcctggt
     7201 gcagcccggc ggcagcctga agctgagctg cgccgtgagc ggcttcagcc tgaccgacta
     7261 cggcgtgaac tgggtgcgcc aggcccccgg ccgcggcctg gagtggatcg gcgtgatctg
     7321 gggcgacggc atcaccgact acaacagcgc cctgaaggac cgcttcatca tcagcaagga
     7381 caacggcaag aacaccgtgt acctgcagat gagcaaggtg cgcagcgacg acaccgccct
     7441 gtactactgc gtgaccggcc tgttcgacta ctggggccag ggcaccctgg tgaccgtgag
     7501 cagctaccca tacgatgttc cagattacgc tggtggaggc ggaggttctg ggggaggagg
     7561 tagtggcggt ggtggttcag gaggcggcgg aagcttggat ccaggtggag gtggaagcgg
     7621 tagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     7681 tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta caaacggaaa
     7741 actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
     7801 cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca tgaaacggca
     7861 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa
     7921 agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa
     7981 tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg gacacaaact
     8041 cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa agaatggaat
     8101 caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac tagcagacca
     8161 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     8221 gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct
     8281 tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca aaggtggagg
     8341 tcggaccggt ggcggtggcg gaggggctag cagatccgaa ctgcccacct gcagctgtct
     8401 tgatcgagtt atacaaaaag acaaaggccc atattataca caccttgggg caggaccaag
     8461 tgttgctgct gtcagggaaa tcatggagaa taggtatggt caaaaaggaa acgcaataag
     8521 gatagaaata gtagtgtaca ccggtaaaga agggaaaagc tctcatgggt gtccaattgc
     8581 taagtgggtt ttaagaagaa gcagtgatga agaaaaagtt ctttgtttgg tccggcagcg
     8641 tacaggccac cactgtccaa ctgctgtgat ggtggtgctc atcatggtgt gggatggcat
     8701 ccctcttcca atggccgacc ggctatacac agagctcaca gagaatctaa agtcatacaa
     8761 tgggcaccct accgacagaa gatgcaccct caatgaaaat cgtacctgta catgtcaagg
     8821 aattgatcca gagacttgtg gagcttcatt ctcttttggc tgttcatgga gtatgtactt
     8881 taatggctgt aagtttggta gaagcccaag ccccagaaga tttagaattg atccaagctc
     8941 tcccttacat gaaaaaaacc ttgaagataa cttacagagt ttggctacac gattagctcc
     9001 aatttataag cagtatgctc cagtagctta ccaaaatcag gtggaatatg aaaatgttgc
     9061 ccgagaatgt cggcttggca gcaaggaagg tcgtcccttc tctggggtca ctgcttgcct
     9121 ggacttctgt gctcatcccc acagggacat tcacaacatg aataatggaa gcactgtggt
     9181 ttgtacctta actcgagaag ataaccgctc tttgggtgtt attcctcaag atgagcagct
     9241 ccatgtgcta cctctttata agctttcaga cacagatgag tttggctcca aggaaggaat
     9301 ggaagccaag atcaaatctg gggccatcga ggtcctggca ccccgccgca aaaaaagaac
     9361 gtgtttcact cagcctgttc cccgttctgg aaagaagagg gctgcgatga tgacagaggt
     9421 tcttgcacat aagataaggg cagtggaaaa gaaacctatt ccccgaatca agcggaagaa
     9481 taactcaaca acaacaaaca acagtaagcc ttcgtcactg ccaaccttag ggagtaacac
     9541 tgagaccgtg caacctgaag taaaaagtga aaccgaaccc cattttatct taaaaagttc
     9601 agacaacact aaaacttatt cgctgatgcc atccgctcct cacccagtga aagaggcatc
     9661 tccaggcttc tcctggtccc cgaagactgc ttcagccaca ccagctccac tgaagaatga
     9721 cgcaacagcc tcatgcgggt tttcagaaag aagcagcact ccccactgta cgatgccttc
     9781 gggaagactc agtggtgcca atgcagctgc tgctgatggc cctggcattt cacagcttgg
     9841 cgaagtggct cctctcccca ccctgtctgc tcctgtgatg gagcccctca ttaattctga
     9901 gccttccact ggtgtgactg agccgctaac gcctcatcag ccaaaccacc agccctcctt
     9961 cctcacctct cctcaagacc ttgcctcttc tccaatggaa gaagatgagc agcattctga
    10021 agcagatgag cctccatcag acgaacccct atctgatgac cccctgtcac ctgctgagga
    10081 gaaattgccc cacattgatg agtattggtc agacagtgag cacatctttt tggatgcaaa
    10141 tattggtggg gtggccatcg cacctgctca cggctcggtt ttgattgagt gtgcccggcg
    10201 agagctgcac gctaccactc ctgttgagca ccccaaccgt aatcatccaa cccgcctctc
    10261 ccttgtcttt taccagcaca aaaacctaaa taagccccaa catggttttg aactaaacaa
    10321 gattaagttt gaggctaaag aagctaagaa taagaaaatg aaggcctcag agcaaaaaga
    10381 ccaggcagct aatgaaggtc cagaacagtc ctctgaagta aatgaattga accaaattcc
    10441 ttctcataaa gcattaacat taacccatga caatgttgtc accgtgtccc cttatgctct
    10501 cacacacgtt gcggggccct ataaccattg ggtctgagcg gccgcgactc tagatcataa
    10561 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    10621 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    10681 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10741 attctagttg tggtttgtcc aaactcatca atgtatcttg gcgcgcctgt acaaaaaagc
    10801 aggctttaaa ggaaccaatt cagtcgactg gatccggtac caaggtcggg caggaagagg
    10861 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
    10921 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
    10981 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
    11041 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttaagt taaaataagg
    11101 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc tagacccagc
    11161 tttcttgtac aaagttggca ttaggcgcgc caaggcgtaa attgtaagcg ttaatatttt
    11221 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
    11281 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
    11341 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
    11401 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
    11461 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
    11521 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
    11581 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
    11641 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    11701 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11761 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
    11821 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
    11881 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
    11941 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
    12001 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    12061 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    12121 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
    12181 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
    12241 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
    12301 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
    12361 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
    12421 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
    12481 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
    12541 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
    12601 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
    12661 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
    12721 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
    12781 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
    12841 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
    12901 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
    12961 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
    13021 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
    13081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
    13141 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
    13201 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
    13261 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
    13321 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
    13381 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
    13441 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    13501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    13561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    13621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    13681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    13741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    13801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    13861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    13921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    13981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    14041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    14101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    14161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    14221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
    14281 cat
//
